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Ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) methods were developed to measure intact IGF-1 protein and IGF-1 trypsin peptides using a triple quadrupole mass spectrometer. Five deer-specific and five human-specific multiple-reaction monitoring (MRM) transitions for intact IGF-1were measured as well as six deer-specific and seven human-specific MRM transitions for an IGF-1 trypsin peptide. Results: The peak area from each MRM transition was used to calculate the product ion ratios relative to the most abundant transition, how to cut 100 sheets of paper. Product ion ratios measured in the supplements were matched to ratios measured in purified protein standards.
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